In the "Impossible Let's Play" series, Markiplier endures physical challenges while he plays a game. These challenges often causes him pain as he is shocked, pinched, or submerged in ice water as he plays. They are not caused by the game themselves but by objects that Mark uses while he plays, like an ice bath or hot sauce.
|Cinnamon Challenge||Actually was recorded in April 26, 2012 on old channel to celebrate first 1000 subscribers milestone. Markiplier just eat one spoon of cinnamon. Although there is no game, it is the original Markiplier's challenge video.||Febuary 1, 2013|||
|Treadmill Challenge||Markiplier plays Surgeon Simulator 2013 while using a treadmill.||April 17, 2014|||
|Hot Sauce Challenge||Markiplier plays Sonic Unfair after drinking the 'Hottest Sauce In The Universe'.||April 20, 2014|||
|Electric Shock Challenge||Markiplier plays with the Don't Let Go with Oculus Rift while being shocked.||April 25, 2014|||
|Clothespin Challenge||Markiplier plays Sonic Unfair with clothespins being added to his face after each in-game death.||May 24, 2014|||
|Ice Bath challenge||Markiplier plays Surgeon Simulator 2013 while sitting in a bath tub full of water and ice.||June 19, 2014|||
|Wax Challenge||Markiplier plays The Impossible Quiz Book with his legs being waxed each time he loses.||July 8, 2014|||
|Make-up Challenge||Markiplier plays The Impossible Game while applying make-up to his face.||July 18, 2014|||
|Wasabi Challenge||Markiplier plays Whack Your Ex and eats small amounts of wasabi before each kill options.||September 5, 2014|||
|Muscule Spasm Challenge||Markiplier plays Disney Fantasia Music Evolved while his arms connected to electric shock wires.||October 25, 2014|||
|Sick Day + Hot Sauce + I Am Bread CHALLENGE!!||While feeling sick, Markiplier plays I Am Bread after consuming a small portion of a 5 million scoville hot sauce.||February 20, 2015|||
- The series is meant to push Mark's physical limit so that he can provide new content to his viewers.